HLA antigens and juvenile onset spondyloarthritides: negative association with non-B27 alleles.

OBJECTIVE To describe the association between HLA-B and HLA-DR genes and juvenile onset spondyloarthritides (SpA) in Mexicans. METHODS The study included 66 consecutive patients with SpA (45 with ankylosing spondylitis (AS) and 21 with undifferentiated SpA) and 99 non-related healthy controls. The HLA-A, -B and DR alleles were detected by the polymerase chain reaction with the sequence-specific primers technique. Statistical methods included the Mantel-Haenzel chi2 test, Fisher's exact test, and Woolf method for odds ratio (OR). RESULTS The frequency of HLA-B27 was significantly increased in the whole group (pC < 10(-3), OR = 53.0, aetiological fraction = 51%), particularly in AS (pC < 10(-3), OR = 67.42, aetiological fraction 57%). In contrast, the frequencies of HLA-B44, and HLA-B14 were significantly decreased. Also, a weak negative association HLA-DR5 (p < 0.05) was found. CONCLUSION Apart from an expected significant association between HLA-B27 and juvenile-onset SpA, particularly AS, we found negative associations with HLA-B44, B14, and DR5. There was also a trend for HLA-B15 and DR1 associations with SpA.